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TEST RESULTS
This section covers results for laboratory based test as well as evaluations conducted in real environments.
Anti-viral test
The laboratory test documented in the table below proves the efficiency of T-Zap as an anti-viral. It shows that T-Zap in its pure form will eliminate the Hong Kong Influenza A type in less than 30 minutes. Without the benefit of the Hydroxyapetite, T-Zap is still effective but needs more time to eliminate the virus.
Test conducted by Bio Medical Science Association of Japan |
Number of live cell determined using MDSK Cell and tested by Black method |
|
After ½ hour |
After 1 hour |
After 2 hours |
Test sample A - T-Zap with Hydroxyapetite |
< 10 |
< 10 |
< 10 |
Test sample B -T-Zap with Hydroxyapetite removed |
14,000 |
5,000 |
<10 |
Control sample |
70,000,000 |
60,000,000 |
50,000,000 |
Viral contamination evaluation
As a second test in real environments, two nursing homes in Japan were evaluated for the number of Influenza cases suffered by their long stay residents / patients. The first facility was a seniors’ home that was not protected with T-Zap products. The second facility was a long-term care center that was protected by T-Zap equipped air purifiers and curtains as well as bed linens. Both facilities housed roughly the same number of patients. The test periods covered the flu seasons – November through March – over 3 consecutive years. The results, shown in the table below, demonstrate a 9 fold lower rate of infection in the T-Zap treated facility than the non treated facility.
Number of influenza cases reported |
2003-2004 |
2004-2005 |
2005 – 2006 |
Total |
Nov to Mar |
Nov to Mar |
Nov to Feb |
17 months |
Control - Seniors’ home without T-Zap |
18 |
76 |
52 |
146 |
Test -
Long term care center with T-Zap |
8 |
8 |
0 |
16 |
Bacteria elimination on coated plates
This laboratory based test used film contact method to determine the number of active cells. Active cells in on the test plates were counted after inoculation and then 24 hours later. One plate was coated with T-Zap, the control was left untreated.
|
At start of test |
After 24 hours |
Control - untreated |
Test – treated with T-Zap |
Number of live MRSA cells on plate |
89,000 |
1,000,000 |
0 |
Air purifier effectiveness test
Two air purifiers were put in the hospital room of a Japanese patient with multi-drug Resistant Staphylococcus aureus (MRSA). One air purifier filter was coated with T-Zap and the other was the air purifier used by the hospital.
After two weeks, 5 samples were removed from each of the filters and used to grow cultures in two sets of Petri dish cultures. The first set was blood agar to test for general germ cultures. The second set was using MRSA isolation medium specifically formulated to grow MRSA cultures.
All cultures were cultivated for 48 hours at 37o Celsius and the resulting bacteria colonies were counted. The table below shows the results. In summary, T-Zap treated filter presented 3.5 times fewer colonies than the control and 2.6 times fewer MRSA specific colonies. These results prove that T-Zap coated air filter captures bacteria and the T-Zap air filter is more effective at eliminating bacteria and specifically MRSA than the normal air purifier filter.
Air purifiers left in room with MRSA patient for 2 weeks |
Blood agar media
(test for general bacteria) |
MRSA specific media
(test for MRSA) |
Control – normal air purifier |
Test –
T-Zap coated filter |
Control – normal air purifier |
Test –
T-Zap coated filter |
Number of colonies after test |
229 |
63 |
73 |
28 |
Anti-bacterial durability test on textile
A Japanese textile manufacturer’s laboratory tested the durability of T-Zap coated cloth. Cloth samples containing no T-Zap were used as the control and two sets of T-Zap cloth samples – one new and the other after 100 normal washing cycles were used as the test. Each cloth was either sprayed with either Staphylococcus arureus (bacteria that cause staph infections) or Escherichia coli (bacteria that cause food poisoning). The test results prove T-Zap remains effective for up to 100 washes, as shown in the table below
Live cell count after culture grown for 18 hours at 37o C |
Staphylococcus arureus ATCC 6538P |
Escherichia coli (IFO 3301) |
Control - Non T-Zap treated cloth |
19,000,000 |
24,000,000 |
Test - T-Zap coated cloth – no wash |
< 20 |
< 20 |
Test – T-Zap coated cloth after 100 washes |
< 20 |
> 20 |
Odor reduction test on textile
This test was conducted by an independent Japanese textile manufacturer. A solution of 2.5% ammonia was sprayed on a towel coated with T-Zap and on a non-treated towel. Parts per million density measurements were then taken after 2 hours.
|
Parts per million concentration after 2 hours |
Control - Untreated towel |
100 |
Test - T-Zap coated towel |
2 |
Anti-odor durability test on textile
This test was conducted by an independent Japanese textile manufacturer. Head bands and socks were worn by volunteers during a strenuous exercise routine and sweat smell tests were conducted upon completion of the session.
|
Head bands |
Socks |
Control - Non T-Zap treated |
Strong smell |
Strong smell |
Test A - T-Zap treated – no wash |
Almost no smell |
Almost no smell |
Test B - T-Zap treated after 20 washes |
Almost no smell |
Almost no smell |
Odor reduction test in paint
This test was conducted by the Japanese Paint Manufacturer Association. They sprayed 2 sets of boards, one with Ammonia concentrate and the other with Acetaldehyde. One set of boards was painted with standard water-based wood paint and the other painted with T-Zap paint. The parts per million concentration where then tested on each board after two hours. The results clearly show that T-Zap additive decomposes both Ammonia and Acetaldehyde.
|
Ammonia concentration in pars per million |
Acetaldehyde concentration in parts per million |
Start of test – both boards |
100 |
100 |
Control – board without T-Zap additive, after 2 hours |
83 |
79 |
Test – board with T-Zap additive, after 2 hours |
6 |
7 |
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